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<font size="4" class="">Dear Michael, </font>
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<div class=""><font size="4" class="">It looks that you already got quite far! I agree that the DTF values do not make much sense.</font></div>
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</font></div>
<div class=""><font size="4" class="">a few pointers:</font></div>
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</font></div>
<div class=""><font size="4" class="">-I would use some regularisation for the MNE reconstruction.</font></div>
<div class=""><font size="4" class="">-I would check whether the individual trials after source reconstruction yield meaningful values in the ‘mom’-field. Particularly, are the different trials different? Do the time series look meaningful to begin with (both
in terms of signal magnitude and ’shape’)? The reason I ask, is that I am not fully sure whether the ‘rawtrial’ option is behaving according to your expectations.</font></div>
<div class=""><font size="4" class="">-Another way for getting the mom would be to use cfg.keepfilter in ft_sourceanalysis, and left-multiply each dipole location’s ’spatial filter’ (in sourceEEG.avg.filter) with a dataEEG.trial matrix.</font></div>
<div class=""><font size="4" class="">-IMPORTANT: don’t use the ‘pow’ for the subsequent spectral analysis and connectivity estimation. You should work with the mom.</font></div>
<div class=""><font size="4" class="">-In order to get a ‘parcellated’ representation of your source level data, I recommend to use ft_sourceparcellate, which should achieve the parcellation you performed by hand (with the binary masks).</font></div>
<div class=""><font size="4" class="">-The parcellation operation can be an average of the mom across the dipoles that belong to a parcel, but an alternative might be the the 1st principal component. The advantage of the latter would be that the per-parcel
signal will be reduced to a single time series (rather then 3 x/y/z orientation time courses), which is convenient (and required) for the interpretation of the subsequent computations.</font></div>
<div class=""><font size="4" class="">-You may want to think a bit about the depth weighting, since the MNE favors superficial locations in terms of amplitude, and this will also percolate to the way the parcellated time series are computed.</font></div>
<div class=""><font size="4" class="">-IMPORTANT: using ‘dtf’ without any additional specification of parameters is probably not what you want here. The reason is that under the hood a multivariate factorization of the spectral matrix is performed (at least:
an attempt is made), which will miserably fail in your case. The reason for this failure (thinking a bit more about this, I am quite surprised that it worked to begin with) is the fact that you ask for a factorization of a size NparcelxNparcel(xfrequency bins)
matrix, where the effective rank of the matrix is less than the number of channels in your EEG array. Even if Nparcel is less than the number of EEG electrodes, this factorization will be very poorly estimated and nonsensical. I’d recommend using ‘granger’
as a method, in combination with cfg.granger.sfmethod = ‘bivariate’. This results in a parcel-pairwise factorization (which is hopefully stable most of the time). I am not a fan of dtf/pdc anyway given that the measures are claimed to provide estimates unproblematic
estimates that are interpretable without any caveats.</font></div>
<div class=""><font size="4" class="">-ALSO IMPORTANT: if you go for a granger-like measure (granger/dtf/pdc), in the spectral analysis you should probably use a decent amount of zero padding (cfg.pad) and sufficient tapsmofrq in order to get spectral data
that stands a chance to get a stable factorization.</font></div>
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</font></div>
<div class=""><font size="4" class="">So, no definitive answers unfortunately, but perhaps some food for thought that might get you closer to a good result.</font></div>
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</font></div>
<div class=""><font size="4" class="">Good luck and keep up the good work,</font></div>
<div class=""><font size="4" class=""><br class="">
</font></div>
<div class=""><font size="4" class="">Jan-Mathijs</font></div>
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<div style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">
<font size="4" class="">J.M.Schoffelen, MD PhD<br class="">
Associate PI, VIDI-fellow - PI, language in interaction<br class="">
Telephone: +31-24-3614793</font></div>
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<font size="4" class="">Physical location: room 00.028</font></div>
<div style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">
<font size="4" class="">Donders Centre for Cognitive Neuroimaging, Nijmegen, The Netherlands<br class="">
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<blockquote type="cite" class="">
<div class="">On 6 May 2020, at 22:34, Michael Glassen - Biomedical Engineer <<a href="mailto:MGlassen@kesslerfoundation.org" class="">MGlassen@kesslerfoundation.org</a>> wrote:</div>
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Hi all,<o:p class=""></o:p></p>
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<o:p class=""> </o:p></p>
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I am having issues using fieldtrip for source reconstruction and connectivity analysis with preprocessed EEG data. I believe the issue is occurring when I try to project my sources to specific ROIs before getting the connectivity. I have outlined my pipeline
below:<o:p class=""></o:p></p>
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<o:p class=""> </o:p></p>
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-Because I do not have subject MRIs, I am using a template headmodel and sourcemodel that come included with fieldtrip<o:p class=""></o:p></p>
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- headmodel = standard_bem.mat<o:p class=""></o:p></p>
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-sourcemodel = cortex_8196.surf.gii<o:p class=""></o:p></p>
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<o:p class=""> </o:p></p>
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-After importing EEG data, I project the EEG electrodes to the scalp using the code below:<o:p class=""></o:p></p>
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<span class="Apple-converted-space"> </span><span style="font-size: 9pt; font-family: "Courier New"; color: blue;" class="">if</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><span class="Apple-converted-space"> </span>strcmp(headmodel.type,<span class="Apple-converted-space"> </span></span><span style="font-size: 9pt; font-family: "Courier New"; color: rgb(160, 32, 240);" class="">'bemcp'</span><span style="font-size: 9pt; font-family: "Courier New";" class="">)</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
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<span style="font-size: 9pt; font-family: "Courier New";" class=""> scalp_index = 3;</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
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<span style="font-size: 9pt; font-family: "Courier New"; color: blue;" class="">elseif</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><span class="Apple-converted-space"> </span>strcmp(headmodel.type,<span class="Apple-converted-space"> </span></span><span style="font-size: 9pt; font-family: "Courier New"; color: rgb(160, 32, 240);" class="">'dipoli'</span><span style="font-size: 9pt; font-family: "Courier New";" class="">)</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
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<span style="font-size: 9pt; font-family: "Courier New";" class=""> scalp_index = 1;</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
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<span style="font-size: 9pt; font-family: "Courier New"; color: blue;" class="">end</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
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<span style="font-size: 9pt; font-family: "Courier New"; color: blue;" class=""></span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
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<span style="font-size: 9pt; font-family: "Courier New";" class="">cfg = [];</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
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<span style="font-size: 9pt; font-family: "Courier New";" class="">cfg.method =<span class="Apple-converted-space"> </span></span><span style="font-size: 9pt; font-family: "Courier New"; color: rgb(160, 32, 240);" class="">'project'</span><span style="font-size: 9pt; font-family: "Courier New";" class="">;<o:p class=""></o:p></span></div>
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<span style="font-size: 9pt; font-family: "Courier New";" class="">cfg.headshape = headmodel.bnd(scalp_index);</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
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<span style="font-size: 9pt; font-family: "Courier New"; color: forestgreen;" class=""> </span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
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<span style="font-size: 9pt; font-family: "Courier New";" class="">eegData.elec = ft_electroderealign(cfg, eegData.elec);<o:p class=""></o:p></span></div>
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<span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""> </o:p></span></div>
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<span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""> </o:p></span></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
-I then calculate covariance on a trial basis and calculate the leadfield matrix:<o:p class=""></o:p></div>
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<o:p class=""> </o:p></div>
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<span class="Apple-converted-space"> </span><span style="font-size: 9pt; font-family: "Courier New";" class="">cfg = [];</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
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<span style="font-size: 9pt; font-family: "Courier New";" class="">cfg.covariance =<span class="Apple-converted-space"> </span></span><span style="font-size: 9pt; font-family: "Courier New"; color: rgb(160, 32, 240);" class="">'yes'</span><span style="font-size: 9pt; font-family: "Courier New";" class="">;</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
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<span style="font-size: 9pt; font-family: "Courier New";" class="">cfg.covariancewindow = [-inf 0];</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
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<span style="font-size: 9pt; font-family: "Courier New";" class="">cfg.keeptrials =<span class="Apple-converted-space"> </span></span><span style="font-size: 9pt; font-family: "Courier New"; color: rgb(160, 32, 240);" class="">'yes'</span><span style="font-size: 9pt; font-family: "Courier New";" class="">;</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
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<span style="font-size: 9pt; font-family: "Courier New";" class="">tlckEeg = ft_timelockanalysis(cfg, eegData);</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
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<span style="font-size: 9pt; font-family: "Courier New";" class=""></span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
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<span style="font-size: 9pt; font-family: "Courier New";" class="">cfg = [];</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
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<span style="font-size: 9pt; font-family: "Courier New";" class="">cfg.elec = tlckEeg.elec; <span class="Apple-converted-space"> </span></span><span style="font-size: 9pt; font-family: "Courier New"; color: forestgreen;" class="">% sensor information</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
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<span style="font-size: 9pt; font-family: "Courier New";" class="">cfg.channel = tlckEeg.label; <span class="Apple-converted-space"> </span></span><span style="font-size: 9pt; font-family: "Courier New"; color: forestgreen;" class="">% the used channels</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
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<span style="font-size: 9pt; font-family: "Courier New";" class="">cfg.grid = sourcemodel; <span class="Apple-converted-space"> </span></span><span style="font-size: 9pt; font-family: "Courier New"; color: forestgreen;" class="">% source points</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
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<span style="font-size: 9pt; font-family: "Courier New";" class="">cfg.headmodel = headmodel; <span class="Apple-converted-space"> </span></span><span style="font-size: 9pt; font-family: "Courier New"; color: forestgreen;" class="">% volume conduction model</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
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<span style="font-size: 9pt; font-family: "Courier New";" class="">cfg.singleshell.batchsize = 5000;<span class="Apple-converted-space"> </span></span><span style="font-size: 9pt; font-family: "Courier New"; color: forestgreen;" class="">% speeds up the computation</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
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<span style="font-size: 9pt; font-family: "Courier New";" class="">leadfield = ft_prepare_leadfield(cfg);</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
<o:p class=""> </o:p></div>
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<span class="Apple-converted-space"> </span><o:p class=""></o:p></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
-Now with the leadfield calculated, I calculate sources on a trial basis here:<o:p class=""></o:p></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
<o:p class=""> </o:p></div>
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<span style="font-size: 9pt; font-family: "Courier New";" class="">cfg = [];</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
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<span style="font-size: 9pt; font-family: "Courier New";" class="">cfg.method =<span class="Apple-converted-space"> </span></span><span style="font-size: 9pt; font-family: "Courier New"; color: rgb(160, 32, 240);" class="">'mne'</span><span style="font-size: 9pt; font-family: "Courier New";" class="">;</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
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<span style="font-size: 9pt; font-family: "Courier New";" class="">cfg.sourcemodel = leadfield;</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
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<span style="font-size: 9pt; font-family: "Courier New";" class="">cfg.headmodel = headmodel;</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
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<span style="font-size: 9pt; font-family: "Courier New";" class="">cfg.mne.prewhiten =<span class="Apple-converted-space"> </span></span><span style="font-size: 9pt; font-family: "Courier New"; color: rgb(160, 32, 240);" class="">'yes'</span><span style="font-size: 9pt; font-family: "Courier New";" class="">;</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
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<span style="font-size: 9pt; font-family: "Courier New";" class="">cfg.mne.lambda = 0;</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
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<span style="font-size: 9pt; font-family: "Courier New";" class="">cfg.mne.scalesourcecov =<span class="Apple-converted-space"> </span></span><span style="font-size: 9pt; font-family: "Courier New"; color: rgb(160, 32, 240);" class="">'yes'</span><span style="font-size: 9pt; font-family: "Courier New";" class="">;</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif; text-indent: 0.5in;" class="">
<span style="font-size: 9pt; font-family: "Courier New";" class="">cfg.rawtrial =<span class="Apple-converted-space"> </span></span><span style="font-size: 9pt; font-family: "Courier New"; color: rgb(160, 32, 240);" class="">'yes'</span><span style="font-size: 9pt; font-family: "Courier New";" class="">;</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif; text-indent: 0.5in;" class="">
<span style="font-size: 9pt; font-family: "Courier New";" class="">sourceEEG = ft_sourceanalysis(cfg,tlckEeg);</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
sourceEEG =<span class="Apple-converted-space"> </span><o:p class=""></o:p></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
<o:p class=""> </o:p></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
struct with fields:<o:p class=""></o:p></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
<o:p class=""> </o:p></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
time: [1×357 double]<o:p class=""></o:p></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
inside: [8196×1 logical]<o:p class=""></o:p></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
pos: [8196×3 double]<o:p class=""></o:p></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
tri: [16384×3 double]<o:p class=""></o:p></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
method: 'rawtrial'<o:p class=""></o:p></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
trial: [1×400 struct]<o:p class=""></o:p></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
df: 400<o:p class=""></o:p></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
cfg: [1×1 struct]<o:p class=""></o:p></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
<o:p class=""> </o:p></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
<o:p class=""> </o:p></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
sourceEEG.trial =<span class="Apple-converted-space"> </span><o:p class=""></o:p></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
<o:p class=""> </o:p></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
1×400 struct array with fields:<o:p class=""></o:p></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
<o:p class=""> </o:p></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
mom<o:p class=""></o:p></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
pow<o:p class=""></o:p></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
noisecov<o:p class=""></o:p></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
<span class="Apple-converted-space"> </span><o:p class=""></o:p></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif; text-indent: 0.5in;" class="">
size(sourceEEG.trial(1).pow)<o:p class=""></o:p></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
<o:p class=""> </o:p></div>
<div style="margin: 0in 0in 0.0001pt 0.5in; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif; text-indent: 0.5in;" class="">
ans =<o:p class=""></o:p></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
<o:p class=""> </o:p></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
8196 357<o:p class=""></o:p></div>
<p class="MsoNormal" style="margin: 0in 0in 8pt; line-height: 15.546667098999023px; font-size: 11pt; font-family: Calibri, sans-serif;">
<o:p class=""> </o:p></p>
<p class="MsoNormal" style="margin: 0in 0in 8pt; line-height: 15.546667098999023px; font-size: 11pt; font-family: Calibri, sans-serif;">
-So at this point I have 400 trials, and for each of these trials I have a time course of source power at 8196 voxels and 357 time points for each of those voxels.<o:p class=""></o:p></p>
<p class="MsoNormal" style="margin: 0in 0in 8pt; line-height: 15.546667098999023px; font-size: 11pt; font-family: Calibri, sans-serif;">
-I then import two atlases, one that includes broddman areas and one that includes left and right hemispheres. I interpolate these atlases onto my source model and get the following:<o:p class=""></o:p></p>
<p class="MsoNormal" style="margin: 0in 0in 8pt; line-height: 15.546667098999023px; font-size: 11pt; font-family: Calibri, sans-serif;">
<span class="Apple-converted-space"> </span><span style="font-size: 9pt; line-height: 12.720000267028809px; font-family: "Calibri Light", sans-serif;" class="">broddman =<o:p class=""></o:p></span></p>
<p class="MsoNormal" style="margin: 0in 0in 8pt 0.5in; line-height: 15.546667098999023px; font-size: 11pt; font-family: Calibri, sans-serif; text-indent: 0.5in;">
<span style="font-size: 9pt; line-height: 12.720000267028809px; font-family: "Calibri Light", sans-serif;" class="">struct with fields:<o:p class=""></o:p></span></p>
<p class="MsoNormal" style="margin: 0in 0in 8pt; line-height: 15.546667098999023px; font-size: 11pt; font-family: Calibri, sans-serif;">
<span style="font-size: 9pt; line-height: 12.720000267028809px; font-family: "Calibri Light", sans-serif;" class=""><o:p class=""> </o:p></span></p>
<p class="MsoNormal" style="margin: 0in 0in 8pt; line-height: 15.546667098999023px; font-size: 11pt; font-family: Calibri, sans-serif;">
<span style="font-size: 9pt; line-height: 12.720000267028809px; font-family: "Calibri Light", sans-serif;" class=""> pos: [8196×3 double]<o:p class=""></o:p></span></p>
<p class="MsoNormal" style="margin: 0in 0in 8pt; line-height: 15.546667098999023px; font-size: 11pt; font-family: Calibri, sans-serif;">
<span style="font-size: 9pt; line-height: 12.720000267028809px; font-family: "Calibri Light", sans-serif;" class=""> tri: [16384×3 double]<o:p class=""></o:p></span></p>
<p class="MsoNormal" style="margin: 0in 0in 8pt; line-height: 15.546667098999023px; font-size: 11pt; font-family: Calibri, sans-serif;">
<span style="font-size: 9pt; line-height: 12.720000267028809px; font-family: "Calibri Light", sans-serif;" class=""> unit: 'm'<o:p class=""></o:p></span></p>
<p class="MsoNormal" style="margin: 0in 0in 8pt; line-height: 15.546667098999023px; font-size: 11pt; font-family: Calibri, sans-serif;">
<span style="font-size: 9pt; line-height: 12.720000267028809px; font-family: "Calibri Light", sans-serif;" class=""> tissue: [8196×1 double] (<b class="">each of these is a value 1-70, corresponding to which atlas entry
the voxel is located in)<o:p class=""></o:p></b></span></p>
<p class="MsoNormal" style="margin: 0in 0in 8pt; line-height: 15.546667098999023px; font-size: 11pt; font-family: Calibri, sans-serif;">
<span style="font-size: 9pt; line-height: 12.720000267028809px; font-family: "Calibri Light", sans-serif;" class=""> tissuelabel: {1×70 cell}<b class="">(ROI labels for the atlas, includes broddman areas not separated by
hemisphere)<o:p class=""></o:p></b></span></p>
<p class="MsoNormal" style="margin: 0in 0in 8pt; line-height: 15.546667098999023px; font-size: 11pt; font-family: Calibri, sans-serif;">
<span style="font-size: 9pt; line-height: 12.720000267028809px; font-family: "Calibri Light", sans-serif;" class=""> cfg: [1×1 struct]<o:p class=""></o:p></span></p>
<p class="MsoNormal" style="margin: 0in 0in 8pt; line-height: 15.546667098999023px; font-size: 11pt; font-family: Calibri, sans-serif; text-indent: 0.5in;">
<span style="font-size: 9pt; line-height: 12.720000267028809px; font-family: "Calibri Light", sans-serif;" class="">hemis2 =<o:p class=""></o:p></span></p>
<p class="MsoNormal" style="margin: 0in 0in 8pt 0.5in; line-height: 15.546667098999023px; font-size: 11pt; font-family: Calibri, sans-serif; text-indent: 0.5in;">
<span style="font-size: 9pt; line-height: 12.720000267028809px; font-family: "Calibri Light", sans-serif;" class="">struct with fields:<o:p class=""></o:p></span></p>
<p class="MsoNormal" style="margin: 0in 0in 8pt 0.5in; line-height: 15.546667098999023px; font-size: 11pt; font-family: Calibri, sans-serif; text-indent: 0.5in;">
<span style="font-size: 9pt; line-height: 12.720000267028809px; font-family: "Calibri Light", sans-serif;" class="">pos: [8196×3 double]<o:p class=""></o:p></span></p>
<p class="MsoNormal" style="margin: 0in 0in 8pt; line-height: 15.546667098999023px; font-size: 11pt; font-family: Calibri, sans-serif;">
<span style="font-size: 9pt; line-height: 12.720000267028809px; font-family: "Calibri Light", sans-serif;" class=""> tri: [16384×3 double]<o:p class=""></o:p></span></p>
<p class="MsoNormal" style="margin: 0in 0in 8pt; line-height: 15.546667098999023px; font-size: 11pt; font-family: Calibri, sans-serif;">
<span style="font-size: 9pt; line-height: 12.720000267028809px; font-family: "Calibri Light", sans-serif;" class=""> unit: 'm'<o:p class=""></o:p></span></p>
<p class="MsoNormal" style="margin: 0in 0in 8pt; line-height: 15.546667098999023px; font-size: 11pt; font-family: Calibri, sans-serif;">
<span style="font-size: 9pt; line-height: 12.720000267028809px; font-family: "Calibri Light", sans-serif;" class=""> tissue: [8196×1 double] (<b class="">each of these is a value 1-7, corresponding to which atlas entry
the voxel is located in)<o:p class=""></o:p></b></span></p>
<p class="MsoNormal" style="margin: 0in 0in 8pt; line-height: 15.546667098999023px; font-size: 11pt; font-family: Calibri, sans-serif;">
<span style="font-size: 9pt; line-height: 12.720000267028809px; font-family: "Calibri Light", sans-serif;" class=""><o:p class=""> </o:p></span></p>
<p class="MsoNormal" style="margin: 0in 0in 8pt; line-height: 15.546667098999023px; font-size: 11pt; font-family: Calibri, sans-serif;">
<span style="font-size: 9pt; line-height: 12.720000267028809px; font-family: "Calibri Light", sans-serif;" class=""> tissuelabel: {1×7 cell}<span class="Apple-converted-space"> </span><b class="">(ROI Labels for hemisphere
atlas, contains 3 Left areas, 3 Right Areas and one inter-hemispheric)<o:p class=""></o:p></b></span></p>
<p class="MsoNormal" style="margin: 0in 0in 8pt; line-height: 15.546667098999023px; font-size: 11pt; font-family: Calibri, sans-serif;">
<span style="font-size: 9pt; line-height: 12.720000267028809px; font-family: "Calibri Light", sans-serif;" class=""> cfg: [1×1 struct]<o:p class=""></o:p></span></p>
<p class="MsoNormal" style="margin: 0in 0in 8pt; line-height: 15.546667098999023px; font-size: 11pt; font-family: Calibri, sans-serif;">
<span style="font-size: 9pt; line-height: 12.720000267028809px; font-family: "Calibri Light", sans-serif;" class=""><o:p class=""> </o:p></span></p>
<p class="MsoNormal" style="margin: 0in 0in 8pt; line-height: 15.546667098999023px; font-size: 11pt; font-family: Calibri, sans-serif;">
<span style="font-size: 9pt; line-height: 12.720000267028809px; font-family: "Calibri Light", sans-serif;" class=""><o:p class=""> </o:p></span></p>
<p class="MsoNormal" style="margin: 0in 0in 8pt; line-height: 15.546667098999023px; font-size: 11pt; font-family: Calibri, sans-serif;">
Now I create a binary mask for each left and right broddman area. For each ROI in the broddman area atlas(1-70), I find all voxels that are located within. This gives me a list of voxels for each broddman area. I then take each of these lists and find which
are in the left hemisphere and which are in the right to get left and right broddman areas.<o:p class=""></o:p></p>
<p class="MsoNormal" style="margin: 0in 0in 8pt; line-height: 15.546667098999023px; font-size: 11pt; font-family: Calibri, sans-serif;">
After these steps I am left with 140 binary masks, (size 1x8196), which equal 1 if the voxel is contained in the specific ROI and 0 if it is not. Using these masks, I extract the time course power for each ROI by taking the mean power of every voxel in the
roi at each time point:<span style="font-size: 9pt; line-height: 12.720000267028809px;" class=""><o:p class=""></o:p></span></p>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
<span style="font-size: 9pt;" class=""> <span class="Apple-converted-space"> </span></span><span style="font-size: 9pt; font-family: "Courier New";" class="">roiData(i,i3,i2) = mean(sourceEEG.trial(i2).pow(Masks{i},i3));<o:p class=""></o:p></span></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
<span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""> </o:p></span></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif; text-indent: 0.5in;" class="">
<span style="font-size: 9pt; font-family: "Courier New";" class="">where i = roi number,<o:p class=""></o:p></span></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
<span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""> </o:p></span></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
<span style="font-size: 9pt; font-family: "Courier New";" class=""> i2 = trial number,<o:p class=""></o:p></span></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
<span style="font-size: 9pt; font-family: "Courier New";" class=""> <span class="Apple-converted-space"> </span><o:p class=""></o:p></span></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
<span style="font-size: 9pt; font-family: "Courier New";" class=""> i3 = time point<o:p class=""></o:p></span></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
<span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""> </o:p></span></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
In order to get this data into fieldtrip format, I put ROI data into an EEGLAB structure along with 6 emg channels that were recorded at the same time and use eeglab2fieldtrip function to have a fieldtrip structure containing the broddman area sources as channels.
(contained in<span class="Apple-converted-space"> </span><i class="">header</i>)<o:p class=""></o:p></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
<o:p class=""> </o:p></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
After this I perform the final step of computing dtf connectivity between each of the source rois and the emg channels with the code below:<o:p class=""></o:p></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
<span class="Apple-converted-space"> </span><span style="font-size: 12pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif; text-indent: 0.5in;" class="">
<span style="font-size: 9pt; font-family: "Courier New";" class="">cfg = [];</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif; text-indent: 0.5in;" class="">
<span style="font-size: 9pt; font-family: "Courier New";" class="">cfg.method =<span class="Apple-converted-space"> </span></span><span style="font-size: 9pt; font-family: "Courier New"; color: rgb(160, 32, 240);" class="">'mtmfft'</span><span style="font-size: 9pt; font-family: "Courier New";" class="">;</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif; text-indent: 0.5in;" class="">
<span style="font-size: 9pt; font-family: "Courier New";" class="">cfg.taper =<span class="Apple-converted-space"> </span></span><span style="font-size: 9pt; font-family: "Courier New"; color: rgb(160, 32, 240);" class="">'dpss'</span><span style="font-size: 9pt; font-family: "Courier New";" class="">;</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif; text-indent: 0.5in;" class="">
<span style="font-size: 9pt; font-family: "Courier New";" class="">cfg.output =<span class="Apple-converted-space"> </span></span><span style="font-size: 9pt; font-family: "Courier New"; color: rgb(160, 32, 240);" class="">'fourier'</span><span style="font-size: 9pt; font-family: "Courier New";" class="">;</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif; text-indent: 0.5in;" class="">
<span style="font-size: 9pt; font-family: "Courier New";" class="">cfg.tapsmofrq = 2;</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif; text-indent: 0.5in;" class="">
<span style="font-size: 9pt; font-family: "Courier New";" class="">freq = ft_freqanalysis(cfg, header);</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif; text-indent: 0.5in;" class="">
<span style="font-size: 9pt; font-family: "Courier New";" class="">cfg = [];</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif; text-indent: 0.5in;" class="">
<span style="font-size: 9pt; font-family: "Courier New";" class="">cfg.method =<span class="Apple-converted-space"> </span></span><span style="font-size: 9pt; font-family: "Courier New"; color: rgb(160, 32, 240);" class="">'dtf'</span><span style="font-size: 9pt; font-family: "Courier New";" class="">;</span><span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""></o:p></span></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif; text-indent: 0.5in;" class="">
<span style="font-size: 9pt; font-family: "Courier New";" class="">dtf = ft_connectivityanalysis(cfg, freq);<o:p class=""></o:p></span></div>
<div style="margin: 0in 0in 0.0001pt; line-height: normal; font-size: 11pt; font-family: Calibri, sans-serif; text-indent: 0.5in;" class="">
<span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""> </o:p></span></div>
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<span style="font-size: 9pt; font-family: "Courier New";" class=""><o:p class=""> </o:p></span></div>
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<span style="" class="">The values I get in DTF are extremely low, 10^-20. I am not sure why but I believe it has to do with the way I am projecting source activations onto specific ROIs. Any help or advice on how to change my pipeline is appreciated.<o:p class=""></o:p></span></div>
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Best,<o:p class=""></o:p></div>
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Michael Glassen<o:p class=""></o:p></div>
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_______________________________________________</span><br style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 14px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none;" class="">
<span style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 14px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; float: none; display: inline !important;" class="">fieldtrip
mailing list</span><br style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 14px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none;" class="">
<a href="https://mailman.science.ru.nl/mailman/listinfo/fieldtrip" style="color: rgb(149, 79, 114); text-decoration: underline; font-family: Helvetica; font-size: 14px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" class="">https://mailman.science.ru.nl/mailman/listinfo/fieldtrip</a><br style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 14px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none;" class="">
<a href="https://doi.org/10.1371/journal.pcbi.1002202" style="color: rgb(149, 79, 114); text-decoration: underline; font-family: Helvetica; font-size: 14px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" class="">https://doi.org/10.1371/journal.pcbi.1002202</a></div>
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